{"id":9141,"date":"2025-10-23T13:52:27","date_gmt":"2025-10-23T05:52:27","guid":{"rendered":"https:\/\/hesp.tcu.edu.tw\/?p=9141"},"modified":"2025-12-02T13:54:00","modified_gmt":"2025-12-02T05:54:00","slug":"%e7%94%9f%e7%89%a9%e9%86%ab%e5%ad%b8%e6%9a%a8%e5%b7%a5%e7%a8%8b%e5%ad%b8%e7%b3%bb%e3%80%90%e8%82%b2%e7%a8%ae%e6%8a%80%e8%a1%93%e8%88%87%e6%87%89%e7%94%a8%e3%80%91%e5%88%86%e5%ad%90%e8%82%b2%e7%a8%ae","status":"publish","type":"post","link":"https:\/\/hesp.tcu.edu.tw\/?p=9141","title":{"rendered":"\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb\u3010\u80b2\u7a2e\u6280\u8853\u8207\u61c9\u7528\u3011\u5206\u5b50\u80b2\u7a2e\u7279\u8272\u8ab2\u7a0b"},"content":{"rendered":"<figure id=\"attachment_9142\" aria-describedby=\"caption-attachment-9142\" style=\"width: 1024px\" class=\"wp-caption alignnone\"><img loading=\"lazy\" decoding=\"async\" class=\"size-large wp-image-9142\" src=\"https:\/\/hesp.tcu.edu.tw\/wp-content\/uploads\/2025\/12\/1023\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb\u3010\u80b2\u7a2e\u6280\u8853\u8207\u61c9\u7528\u3011\u5206\u5b50\u80b2\u7a2e\u7279\u8272\u8ab2\u7a0b-1024x768.png\" alt=\"\" width=\"1024\" height=\"768\" srcset=\"https:\/\/hesp.tcu.edu.tw\/wp-content\/uploads\/2025\/12\/1023\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb\u3010\u80b2\u7a2e\u6280\u8853\u8207\u61c9\u7528\u3011\u5206\u5b50\u80b2\u7a2e\u7279\u8272\u8ab2\u7a0b-1024x768.png 1024w, https:\/\/hesp.tcu.edu.tw\/wp-content\/uploads\/2025\/12\/1023\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb\u3010\u80b2\u7a2e\u6280\u8853\u8207\u61c9\u7528\u3011\u5206\u5b50\u80b2\u7a2e\u7279\u8272\u8ab2\u7a0b-300x225.png 300w, https:\/\/hesp.tcu.edu.tw\/wp-content\/uploads\/2025\/12\/1023\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb\u3010\u80b2\u7a2e\u6280\u8853\u8207\u61c9\u7528\u3011\u5206\u5b50\u80b2\u7a2e\u7279\u8272\u8ab2\u7a0b-768x576.png 768w, https:\/\/hesp.tcu.edu.tw\/wp-content\/uploads\/2025\/12\/1023\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb\u3010\u80b2\u7a2e\u6280\u8853\u8207\u61c9\u7528\u3011\u5206\u5b50\u80b2\u7a2e\u7279\u8272\u8ab2\u7a0b.png 1477w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption id=\"caption-attachment-9142\" class=\"wp-caption-text\">\u5716\/\u6587:\u751f\u7269\u91ab\u5b78\u66a8\u5de5\u7a0b\u5b78\u7cfb<\/figcaption><\/figure>\n<p>\u25ce CRISPR\/Cas Technique<br \/>\nTechnical Supports for CRISPR\/Cas Applications<br \/>\nTechnical support for CRISPR\/Cas applications involves using delivery methods like nanoparticles, electroporation, and viral vectors to get the system into cells, along with computational tools for designing guide RNAs and analyzing results. Researchers must optimize parameters for specific cell types to maximize efficiency while minimizing toxicity and off-target effects.<\/p>\n<p>CRISPR\/Cas technology can be used for genetic rescue in extinct or endangered species by precisely editing genomes to fix harmful mutations, increase genetic diversity, and enhance adaptability to environmental changes. For de-extinction, scientists can use a living relative\u2019s genome and CRISPR to edit it with genes from the extinct species to create a hybrid. These techniques offer a more targeted approach than traditional methods, though ethical and practical challenges remain.<\/p>\n<p>\u25ce RNAi technique<br \/>\nGenome-Scale shRNA Libraries<br \/>\nGenome-scale short hairpin RNA (shRNA) libraries use RNA interference (RNAi) to systematically silence thousands of genes simultaneously, allowing researchers to identify genes critical to specific biological processes through loss-of-function screens. This technique uses pooled or arrayed libraries of shRNAs, which are delivered to a population of cells to create a pool of cells, each with a different gene knocked down. The cells are then subjected to a specific selective pressure (e.g., drug treatment, growth condition), and the shRNAs that survive or thrive are identified through sequencing, revealing the genes they targeted.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u25ce CRISPR\/Cas Technique Technical Supports for CRISPR\/Ca [&hellip;]<\/p>\n","protected":false},"author":8,"featured_media":9142,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[35,21,22,44,47],"tags":[],"class_list":["post-9141","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-sdg-17-","category-sdgs-3","category-sdg-4-","category-44","category-47"],"blocksy_meta":[],"_links":{"self":[{"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts\/9141","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9141"}],"version-history":[{"count":1,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts\/9141\/revisions"}],"predecessor-version":[{"id":9143,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/posts\/9141\/revisions\/9143"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=\/wp\/v2\/media\/9142"}],"wp:attachment":[{"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9141"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9141"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/hesp.tcu.edu.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9141"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}